Prism doesn't 'know' these labels are numbers, so doesn't use the value as a X coordinate.FlowJo v10 makes it easy to convert bivariate dot plots to univariate histograms with a click of a button! Also note that the values are equally spread across the X axis. To view your plot as a histogram, simply click the drop-down menu on the left side of the Graph Window and select “Histogram” from the menu. Use histograms to view frequency distribution of your flow data, one parameter at a time. Histograms display a frequency distribution of the data versus fluorescence intensity or some other parameter (e.g. This can be helpful to quickly analyze whether your samples have normal, uniform, bimodal, or other type of distribution. 7-AAD) was used during staining, you can quickly evaluate whether you have high or low numbers of live cells (live cells will be largely 7-AAD negative). In contrast to dot plots or other bivariate displays, there are different types of options and gating tools for histograms. See the sections below for more information about histogram options and gating tools.

Graphpad prism 7 historam